Based on the information in the provided URL, we have the EGFP/EYFP Monoclonal Antibody (MACO0221). This antibody is specifically designed for research involving enhanced green fluorescent protein (EGFP) and enhanced yellow fluorescent protein (EYFP), commonly used in cell labeling and fluorescence microscopy studies. The EGFP/EYFP Monoclonal Antibody is derived from mouse monoclonal antibodies and is highly reactive with samples expressing EGFP and EYFP proteins. It has been validated for applications such as immunofluorescence, flow cytometry, and Western blotting, allowing for efficient detection and analysis of EGFP and EYFP fusion proteins in various experimental settings.
These fluorescent proteins are widely utilized in cell biology and molecular biology research, enabling visualization of cellular processes, protein localization, and gene expression studies. The EGFP/EYFP Monoclonal Antibody provides researchers with a powerful tool for tracking and studying these proteins, facilitating advances in fields such as genetics, developmental biology, and drug discovery. Overall, the EGFP/EYFP Monoclonal Antibody offers a valuable resource for scientists seeking to further their understanding of protein dynamics and cellular function through fluorescence-based techniques.
Product Name:
Anti-EGFP/EYFP Antibody
Product Sku:
MACO0221
Size:
100ul
Host Species:
Mouse
Tested Applications:
ELISA, WB
Recommended Dilutions:
WB:1:500-1:5000
Species Reactivity:
Bacillus cereus
Immunogen:
Purified recombinant EYFP.tag full length expressed in E.coli.
Form:
Liquid
Storage Buffer:
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Purification Method:
Affinity purified
Clonality:
Monoclonal
Isotype:
IgG1
Conjugate:
Non-conjugated
Background:
The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.
Synonyms:
GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein;