The EFS Polyclonal Antibody (CAB16479) is designed for research involving EFS, a protein integral to regulating cell shape and motility. The antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It binds to the EFS protein, allowing for precise detection and analysis in a variety of cell types, making it an ideal tool for studies in cell biology and cancer research.EFS, also known as Embryonal Fyn-associated Substrate, plays a crucial role in the organization and function of the actin cytoskeleton, influencing processes such as cell migration, adhesion, and signaling.
Its involvement in these fundamental cellular functions makes it a key target for research in understanding various physiological and pathological processes. Investigating the function of EFS can provide valuable insights into cellular dynamics and potential therapeutic targets for diseases associated with dysregulated cell motility.
Product Name:
EFS Rabbit Polyclonal Antibody
SKU:
CAB16479
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-190 of human EFS (NP_115835.1).
The protein encoded by this gene is a member of the CAS (CRK-associated substrate) family of adaptor proteins which typically serve as scaffolds for the assembly of larger signaling complexes. These complexes form at the cell surface where integrin binding leads to the subsequent phosphorylation of a CAS protein. Additional binding of SRC family kinases leads to CAS hyperphosphorylation and the creation of binding sites for CRK and other proteins that cause actin cytoskeleton reorganization. This gene plays a role in integrin-mediated cell attachment, spreading, and migration and also plays a role in both normal and malignant cellular transformation. This broadly expressed gene has been shown to play a role in neurite outgrowth and its expression in the thymus and lymphocytes is important for T cell maturation and the development of immunological self-tolerance. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
10278
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse thymus, using EFS Rabbit pAb (CAB16479) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
