The EFHD1 Polyclonal Antibody (PAC061678) offered by AssayGenie is a valuable tool for researchers studying EFHD1, a calcium-binding protein involved in various cellular processes. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding to the EFHD1 protein, researchers can accurately detect and analyze EFHD1 expression in different cell types, making it an ideal choice for studies in cell biology and neurodegenerative diseases.
EFHD1, a member of the EF-hand calcium-binding protein family, is known to play a role in cell signaling, cytoskeletal organization, and synaptic transmission. Its involvement in calcium-dependent processes makes it a potential target for investigating diseases such as Alzheimer's and Parkinson's. By understanding the function of EFHD1, researchers can uncover new insights into the mechanisms underlying these neurological disorders and potentially develop novel therapeutic strategies.
Western Blot. Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate. All lanes: EFHD1 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 27, 17 kDa. Observed band size: 27 kDa.
Immunofluorescence staining of A549 cells with PACO61678 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO61678 diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production. May play a role in neuronal differentiation (By similarity).
Synonyms:
EF-hand domain-containing protein D1, EF-hand domain-containing protein 1, Swiprosin-2, EFHD1, SWS2, PP3051
UniProt Protein Function:
EFHD1: 2 isoforms of the human protein are produced by alternative splicingProtein type: MitochondrialChromosomal Location of Human Ortholog: 2q37.1
UniProt Protein Details:
NCBI Summary:
EFHD1 is an EF-hand domain-containing protein that displays increased expression during neuronal differentiation (Tominaga and Tomooka, 2002 [PubMed 12270117]).[supplied by OMIM, Mar 2008]
