The EEF1D Polyclonal Antibody (CAB2509) is a vital tool for researchers studying EEF1D, a protein involved in protein synthesis and cellular signaling. This antibody is produced in rabbits and has been extensively validated for use in Western blot applications, showing high reactivity with human samples. By specifically binding to EEF1D, this antibody allows for the accurate detection and analysis of the protein in various cell types.EEF1D, also known as elongation factor 1 delta, plays a crucial role in the elongation phase of protein synthesis and has been implicated in various cellular processes. Its role in translation regulation and signal transduction pathways make it a key player in cancer research, neurodegenerative diseases, and other disorders involving aberrant protein synthesis.
By studying EEF1D, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the EEF1D Polyclonal Antibody (CAB2509) is an essential tool for researchers investigating the role of EEF1D in cellular processes and disease pathogenesis. Its high specificity and reactivity make it an ideal choice for studies in molecular biology, cancer biology, and beyond. Order now and accelerate your research with this reliable antibody.
Product Name:
EEF1D Rabbit Polyclonal Antibody
SKU:
CAB2509
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
EF1D; EF-1D; FP1047; EEF1D
Positive Sample:
MCF7,Mouse testis,jurkat,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
31kDa
Observed MW:
40kDa/35kDa/40kDa
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
Purification Method:
Affinity purification
Gene ID:
1936
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using EEF1D Rabbit pAb (CAB2509) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
