The EEA1 Rabbit Monoclonal Antibody (CAB5057) is a cutting-edge tool for researchers studying endosomal trafficking and autophagy processes. This antibody is specifically designed to target the Early Endosome Antigen 1 (EEA1) protein, a key marker for early endosomes involved in intracellular transport pathways.Raised in rabbits, this monoclonal antibody offers high specificity and sensitivity when detecting EEA1 in various cell types and tissues. Validated for use in techniques such as immunofluorescence and immunohistochemistry, the CAB5057 antibody allows for precise visualization and analysis of EEA1 localization and distribution within the cell.The EEA1 protein is essential for endosomal fusion events and plays a crucial role in regulating membrane dynamics and cargo sorting.
Dysfunction in EEA1-mediated processes has been linked to various diseases, including neurodegenerative disorders and infectious diseases. By utilizing the CAB5057 antibody, researchers can gain valuable insights into the molecular mechanisms underlying these pathological conditions and potentially identify new therapeutic targets.Overall, the EEA1 Rabbit Monoclonal Antibody (CAB5057) is a powerful tool for dissecting the intricate machinery of endosomal trafficking and autophagy pathways, offering researchers a deeper understanding of cellular processes essential for maintaining homeostasis and health.
Product Name:
EEA1 Rabbit Monoclonal Antibody
SKU:
CAB5057
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EEA1 (Q15075).
Cytoplasm, Early endosome membrane, Peripheral membrane protein.
Calculated MW:
162kDa
Observed MW:
170kDa
Enables 1-phosphatidylinositol binding activity; GTP-dependent protein binding activity; and protein homodimerization activity. Involved in endocytosis; vesicle fusion; and viral RNA genome replication. Located in cytosol and early endosome. Is extrinsic component of plasma membrane.
Purification Method:
Affinity purification
Gene ID:
8411
Clone Number:
ARC1250
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EEA1 Rabbit mAb (CAB5057) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
