The EEA1 Polyclonal Antibody (CAB0592) is a valuable tool for researchers studying endosomal trafficking and membrane dynamics. This antibody, raised in rabbits, is highly specific to the Early Endosome Antigen 1 (EEA1) protein and has been rigorously validated for use in various applications, including immunofluorescence and immunohistochemistry.EEA1 is a key component of the early endosome compartment, playing a crucial role in sorting and trafficking of endocytic vesicles. Its involvement in membrane fusion events and signaling cascades makes it a target of interest in studies of cellular transport processes and intracellular trafficking pathways. The EEA1 Polyclonal Antibody enables precise detection and visualization of EEA1 protein in different cell types and tissues, providing valuable insights into endosomal dynamics and organelle interactions.
Researchers in the fields of cell biology, molecular biology, and vesicle trafficking will find the EEA1 Polyclonal Antibody to be an essential tool for investigating the intricate mechanisms of endocytic pathways and understanding the regulation of membrane dynamics within the cell. Its high specificity and sensitivity make it a reliable reagent for detailed analysis of EEA1 localization and function, paving the way for novel discoveries in the field of cell biology.
Product Name:
EEA1 Rabbit Polyclonal Antibody
SKU:
CAB0592
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1182-1411 of human EEA1 (NP_003557.2).
Cytoplasm, Early endosome membrane, Peripheral membrane protein.
Calculated MW:
162kDa
Observed MW:
175kDa
Enables 1-phosphatidylinositol binding activity; GTP-dependent protein binding activity; and protein homodimerization activity. Involved in endocytosis; vesicle fusion; and viral RNA genome replication. Located in cytosol and early endosome. Is extrinsic component of plasma membrane.
Purification Method:
Affinity purification
Gene ID:
8411
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using EEA1 Rabbit pAb (CAB0592) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
