The EDEM2 Polyclonal Antibody (CAB17181) is a valuable tool for researchers studying EDEM2, a protein involved in endoplasmic reticulum-associated degradation (ERAD) pathway. Raised in rabbits, this antibody is highly specific for detecting EDEM2 in human samples and is validated for use in Western blot applications. By binding to the EDEM2 protein, this antibody allows for precise detection and analysis in various cell types, making it an essential asset for studies in cell biology and protein degradation mechanisms.EDEM2 is a key player in quality control processes within the ERAD pathway, where it helps identify and target misfolded proteins for degradation.
Dysregulation of EDEM2 function has been implicated in various diseases, including neurodegenerative disorders and metabolic syndromes. Therefore, understanding the role of EDEM2 is crucial for unraveling the underlying mechanisms of these conditions and developing targeted therapies.By utilizing the EDEM2 Polyclonal Antibody (CAB17181), researchers can gain insights into the function and regulation of EDEM2, paving the way for advancements in the field of protein quality control and potential therapeutic interventions for ERAD-associated diseases.
Product Name:
EDEM2 Rabbit Polyclonal Antibody
SKU:
CAB17181
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 399-578 of human EDEM2 (NP_060687.2).
endoplasmic reticulum, endoplasmic reticulum lumen, endoplasmic reticulum quality control compartment
Calculated MW:
65kDa
Observed MW:
70kDa
In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).
Purification Method:
Affinity purification
Gene ID:
55741
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EDEM2 Rabbit pAb (CAB17181) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
