The ECH1 Polyclonal Antibody (CAB12944) is specifically designed for research involving ECH1, an enzyme involved in fatty acid metabolism and peroxisomal beta-oxidation. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the ECH1 protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal tool for studies in metabolism, lipid metabolism disorders, and related diseases.ECH1 plays a crucial role in fatty acid oxidation, a process essential for energy production and maintaining metabolic balance.
Dysregulation of ECH1 activity has been linked to metabolic disorders such as diabetes, obesity, and cardiovascular diseases. Research on ECH1 is vital for understanding the mechanisms underlying these conditions and developing targeted therapies to treat them effectively.By utilizing the ECH1 Polyclonal Antibody (CAB12944), researchers can further explore the role of ECH1 in metabolism and disease, advancing our understanding of metabolic pathways and potentially leading to the development of new therapeutic strategies for metabolic disorders.
Product Name:
ECH1 Rabbit Polyclonal Antibody
SKU:
CAB12944
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).
This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
Purification Method:
Affinity purification
Gene ID:
1891
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ECH1 Rabbit pAb (CAB12944) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
