The ECE1 Polyclonal Antibody is a vital tool for research involving Endothelin-Converting Enzyme 1 (ECE1), an important enzyme involved in the regulation of endothelin, a potent vasoconstrictor peptide. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the ECE1 enzyme, this antibody allows for the detection and analysis of ECE1 expression in various cell types, making it ideal for studies in cardiovascular research and hypertension.ECE1 plays a crucial role in the conversion of big endothelin to active endothelin, a process that has implications in the development of cardiovascular diseases such as hypertension and heart failure.
Understanding the function and regulation of ECE1 is essential for developing targeted therapies that could potentially modulate endothelin levels and improve cardiovascular health. The ECE1 Polyclonal Antibody provides researchers with a valuable tool for investigating the role of ECE1 in normal physiological processes and disease states, paving the way for potential therapeutic interventions.
Product Name:
ECE1 Polyclonal Antibody
SKU:
CAB21824
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 670-770 of human ECE1 (NP_001388.1).
Cell membrane, Single-pass type II membrane protein.
Calculated MW:
87kDa
Observed MW:
125-135kDa
The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Purification Method:
Affinity purification
Gene ID:
1889
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using ECE1 Rabbit pAb(CAB21824) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time:180s.
