The EARS2 Polyclonal Antibody (CAB14959) is a valuable tool for researchers studying EARS2, an essential mitochondrial enzyme involved in tRNA processing and mitochondrial protein translation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.EARS2 plays a crucial role in maintaining mitochondrial function and cellular metabolism, making it a key player in energy production and overall cell health. Dysregulation of EARS2 has been linked to various mitochondrial disorders and diseases, highlighting the importance of understanding its function and regulation.
By targeting the EARS2 protein, this polyclonal antibody allows for reliable detection and analysis of EARS2 levels in different cell types, enabling researchers to explore its role in mitochondrial biology and disease pathogenesis. With its specificity and sensitivity, the EARS2 Polyclonal Antibody is a valuable tool for studies in mitochondrial disorders, metabolic diseases, and related research areas.
Product Name:
EARS2 Rabbit Polyclonal Antibody
SKU:
CAB14959
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-220 of human EARS2 (NP_001295140.1).
This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
124454
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse kidney, using EARS2 Rabbit pAb (CAB14959) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
