The E3 (Estriol) ELISA Kit is designed for the quantitative detection of Estriol (E3) levels in various biological samples. Estriol is a primary estrogen that is predominantly produced during pregnancy and plays a significant role in fetal development and maternal health. It is a crucial biomarker involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. Accurate measurement of E3 is necessary for understanding its involvement in pregnancy-related mechanisms, reproductive health, and for monitoring relevant therapeutic interventions.
Assay Genie's E3 (Estriol) ELISA Kit offers exemplary sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's E3 (Estriol) ELISA Kit for precise and reliable detection of this crucial biomarker in your studies.
Product Name:
E3 (Estriol) ELISA Kit
SKU:
AEES00015
Target:
E3 (Estriol)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
3.70 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E3. During the reaction, E3 in the sample or standard competes with a fixed amount of E3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
80-91
88-103
85-108
Average (%)
85
90
94
1:4
Range (%)
82-95
87-94
80-111
Average (%)
88
90
92
1:8
Range (%)
84-101
82-89
83-95
Average (%)
93
85
87
1:16
Range (%)
87-106
89-114
84-96
Average (%)
92
99
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
81-95
85
EDTA plasma (n=5)
86-98
89
Cell culture media (n=5)
85-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
30.63
80.77
200.24
33.54
82.78
205.73
Standard deviation
2.55
5.84
10.85
3.24
6.83
14.85
C V (%)
8.34
7.23
5.42
9.67
8.25
7.22
Sample type &Sample volume:
serum and plasma; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of E3 concentrations in serum and plasma.
Specificity:
This kit recognizes E3 in samples. No significant cross-reactivity or interference between E3 and analogues was observed.
