Anti-E2F8 Antibody (CAB1135)

Antibody Name:	Anti-E2F8 Antibody
Antibody SKU:	CAB1135
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human E2F8 (NP_078956.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human E2F8 (NP_078956.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MENE KENL FCEP HKRG LMKT PLKE STTA NIVL AEIQ PDFG PLTT PTKP KEGS QGEP WTPT ANLK MLIS AVSP EIRN RDQK RGLF DNRS GLPE AKDC IHEH LSGD EFEK SQPS RKEK SLGL LCHK FLAR YPNY PNPA VNND ICLD EVAE ELNV ERRR IYDI VNVL ESLH MVSR LAKN RYTW HGRH NLNK TLGT LKSI GEEN KYAE QIMM IKKK EYEQ EFDF IKSY SIED HIIK SNTG PNGH
Gene ID:	79733
Uniprot:	A0AVK6
Cellular Location:	Nucleus
Calculated MW:	94kDa
Observed MW:	82kDa

Synonyms:	E2F8, E2F-8
Background:	This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	E2F8: Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. Belongs to the E2F/DP family.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 11p15.1 Cellular Component: transcription factor complex; nucleus Molecular Function:protein binding; protein homodimerization activity; transcription corepressor activity; transcription factor activity Biological Process: cell proliferation; transcription, DNA-dependent; positive regulation of DNA endoreduplication; positive regulation of transcription from RNA polymerase II promoter; sprouting angiogenesis; cell cycle comprising mitosis without cytokinesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cytokinesis; placenta development
NCBI Summary:	This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012]
UniProt Code:	A0AVK6
NCBI GenInfo Identifier:	121949436
NCBI Gene ID:	79733
NCBI Accession:	A0AVK6.1
UniProt Secondary Accession:	A0AVK6,Q2VPJ3, Q3C1U6, Q5BKY4, Q8N340, Q9H5M0, A8K9H3
UniProt Related Accession:	A0AVK6
Molecular Weight:	867
NCBI Full Name:	Transcription factor E2F8
NCBI Synonym Full Names:	E2F transcription factor 8
NCBI Official Symbol:	E2F8
NCBI Official Synonym Symbols:	E2F-8
NCBI Protein Information:	transcription factor E2F8; E2F family member 8
UniProt Protein Name:	Transcription factor E2F8
Protein Family:	Transcription factor
UniProt Gene Name:	E2F8
UniProt Entry Name:	E2F8_HUMAN

Western blot analysis of extracts of various cell lines, using E2F8 antibody (CAB1135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).

Antibodies

E2F8 Antibody (PACO08976)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)