Anti-E2F6 Antibody (CAB6151)

Antibody Name:	Anti-E2F6 Antibody
Antibody SKU:	CAB6151
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).

Application:	WB IHC IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100 IP 1:50 - 1:100 ChIP 1:20 - 1:50
Reactivity:	Human, Mouse
Positive Samples:	MCF-7, Jurkat, HeLa, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSQQ RPAR KLPS LLLD PTEE TVRR RCRD PINV EGLL PSKI RINL EDNV QYVS MRKA LKVK RPRF DVSL VYLT RKFM DLVR SAPG GILD LNKV ATKL GVRK RRVY DITN VLDG IDLV EKKS KNHI RWIG SDLS NFGA VPQQ KKLQ EELS DLSA MEDA LDEL IKDC AQQL FELT DDKE NERL AYVT YQDI HSIQ AFHE QIVI AVKA PAET RLDV PAPR EDSI TVHI RSTN GPID VYLC EVEQ GQTS NKRS EGVG TSSS ESTH PEGP EEEE NPQQ SEEL LEVS N
Gene ID:	1876
Uniprot:	O75461
Cellular Location:	Nucleus
Calculated MW:	22kDa/28kDa/31kDa
Observed MW:	37kDa

Synonyms:

E2F6, E2F-6

Background:

This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	E2F6: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. Belongs to the E2F/DP family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 2p25.1 Cellular Component: transcription factor complex; nucleus Molecular Function:DNA binding; transcription factor activity; transcription corepressor activity Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Code:	O75461
NCBI GenInfo Identifier:	7993930
NCBI Gene ID:	1876
NCBI Accession:	O75461.1
UniProt Secondary Accession:	O75461,O60544, Q53QY9, Q6Q9Z6, Q7Z2H6, A8K2Z8, G5E936
UniProt Related Accession:	O75461
Molecular Weight:	28,103 Da
NCBI Full Name:	Transcription factor E2F6
NCBI Synonym Full Names:	E2F transcription factor 6
NCBI Official Symbol:	E2F6
NCBI Official Synonym Symbols:	E2F-6
NCBI Protein Information:	transcription factor E2F6
UniProt Protein Name:	Transcription factor E2F6
Protein Family:	Transcription factor
UniProt Gene Name:	E2F6
UniProt Entry Name:	E2F6_HUMAN

	Western blot analysis of extracts of various cell lines, using E2F6 antibody (CAB6151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using E2F6 antibody (CAB6151) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using E2F6 antibody (CAB6151). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 3ug E2F6 antibody (CAB6151). Western blot was performed from the immunoprecipitate using E2F6 antibody (CAB6151) at a dilition of 1:500.
	Chromatin immunoprecipitation analysis of extracts of HeLa cells, using E2F6 antibody (CAB6151) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Antibodies

Anti-E2F6 Antibody (CAB2718)

Anti-E2F6 Antibody (CAB11546)

E2F6 Antibody (PACO06442)

E2F6 Antibody (PACO08975)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)