The E2F6 Polyclonal Antibody (CAB6151) is a valuable tool for researchers studying the E2F6 protein, a transcription factor involved in cell cycle regulation and cancer development. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the E2F6 protein, researchers can accurately detect and analyze its expression in various cell types, advancing studies in oncology and cell biology.E2F6 is a key player in controlling cell proliferation and differentiation, making it a crucial target for investigation in cancer research. Dysregulation of E2F6 has been linked to tumor growth and progression, highlighting its potential as a therapeutic target for cancer treatment.
By uncovering the mechanisms by which E2F6 functions, researchers can develop novel strategies for cancer therapy and precision medicine approaches.Overall, the E2F6 Polyclonal Antibody (CAB6151) offers researchers a reliable tool for studying the role of E2F6 in cancer biology, cell cycle regulation, and potential therapeutic interventions. Its high specificity and sensitivity make it a valuable asset for advancing our understanding of E2F6's functions in health and disease.
Product Name:
E2F6 Polyclonal Antibody
SKU:
CAB6151
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
WB,1:100 - 1:500 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:
E2F-6; E2F6
Positive Sample:
HeLa,Mouse lung
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
32kDa
Observed MW:
36kDa
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1876
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using E2F6 Rabbit pAb (CAB6151) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
