The E2F6 Polyclonal Antibody (CAB2718) is a valuable tool for researchers studying E2F6, a transcription factor involved in cell cycle regulation and development. This antibody, generated in rabbits, is highly specific to human samples and is ideal for use in Western blot applications. By binding to the E2F6 protein, this antibody enables the detection and analysis of E2F6 expression in various cell types, making it a crucial component of studies in molecular biology and cancer research.E2F6 is known for its role in cell cycle control and its involvement in tumorigenesis, making it a promising target for cancer research.
The ability to accurately detect and quantify E2F6 expression levels is essential for understanding its function in normal and diseased cells, shedding light on potential therapeutic strategies for cancer treatment. Researchers studying cell cycle regulation, development, and cancer biology will benefit from the specificity and reliability of the E2F6 Polyclonal Antibody (CAB2718) in their experiments.
Product Name:
E2F6 Rabbit Polyclonal Antibody
SKU:
CAB2718
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:
E2F-6; E2F6
Positive Sample:
Mouse lung
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
32kDa
Observed MW:
35kDa
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1876
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse lung using E2F6 Rabbit pAb(CAB2718) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime:180s.
