The E2F6 Rabbit Monoclonal Antibody is a powerful tool for researchers studying the E2F6 protein, a key regulator of cell cycle progression and gene expression. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting E2F6 in various biological samples.By targeting the E2F6 protein, this antibody allows for precise detection and analysis in research areas such as cell biology, cancer biology, and developmental biology. E2F6 plays a crucial role in controlling cell proliferation and differentiation, making it an essential molecule to study in the context of tumorigenesis and other diseases.
Utilizing this antibody in techniques like Western blotting, immunohistochemistry, and flow cytometry can provide valuable insights into the function and regulation of E2F6 in cellular processes. Researchers can rely on the E2F6 Rabbit Monoclonal Antibody to enhance their understanding of E2F6 biology and its implications in various pathological conditions.
Product Name:
E2F6 Rabbit Monoclonal Antibody
SKU:
CAB11546
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 110-281 of human E2F6 (NP_937987.2)
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of ChromatinCUT&Tag, 10⁵ cells /1 μg
Synonyms:
E2F-6; E2F6
Positive Sample:
293T,HepG2,RD
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
32kDa
Observed MW:
36kDa
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1876
Clone Number:
ARC0628
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1 μg E2F6 Rabbit mAb (CAB11546), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of E2F6 in representative gene loci (BRCA1), as shown in figure.
