The E2F3 Polyclonal Antibody (CAB8811) is a valuable tool for researchers studying the E2F3 protein, a key regulator of cell cycle progression and proliferation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications, allowing for the detection and analysis of E2F3 expression in various cell types.E2F3 is a transcription factor that plays a critical role in controlling the expression of genes involved in cell division and DNA replication. Dysregulation of E2F3 has been linked to various diseases, including cancer, making it an important target for research in oncology and cell biology.
By using the E2F3 Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of E2F3 in normal and disease states, providing a deeper understanding of its role in cell cycle control and potential therapeutic interventions. This antibody is a valuable tool for studies in cancer biology, cell signaling, and drug development efforts targeting E2F3-related pathways.
Product Name:
E2F3 Rabbit Polyclonal Antibody
SKU:
CAB8811
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human E2F3 (NP_001230005.1).
This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1871
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using E2F3 Rabbit pAb (CAB8811) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
