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Anti-E2F3 Antibody (CAB8811)

SKU:
CAB8811
Product Type:
Antibody
Applications:
WB
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-E2F3 Antibody
Antibody SKU:CAB8811
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human E2F3 (NP_001230005.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human E2F3 (NP_001230005.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SIES LQIH LAST QGPI EVYL CPEE TETH SPMK TNNQ DHNG NIPK PASK DLAS TNSG HSDC SVSM GNLS PLAS PANL LQQT EDQI PSNL EGPF VNLL PPLL Q
Gene ID:1871
Uniprot:O00716
Cellular Location:Nucleus
Calculated MW:37kDa/49kDa
Observed MW:53kDa
Synonyms:E2F3, E2F-3
Background:This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:E2F3: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Belongs to the E2F/DP family.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p22

Cellular Component: nucleoplasm; Golgi apparatus; transcription factor complex; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function:protein binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; mitotic cell cycle

NCBI Summary:This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
UniProt Code:O00716
NCBI GenInfo Identifier:2811006
NCBI Gene ID:1871
NCBI Accession:O00716.1
UniProt Related Accession:O00716
Molecular Weight:
NCBI Full Name:Transcription factor E2F3
NCBI Synonym Full Names:E2F transcription factor 3
NCBI Official Symbol:E2F3  
NCBI Official Synonym Symbols:E2F-3  
NCBI Protein Information:transcription factor E2F3
UniProt Protein Name:Transcription factor E2F3
Protein Family:Transcription factor
UniProt Gene Name:E2F3  
UniProt Entry Name:E2F3_HUMAN
Anti-E2F3 Polyclonal Antibody (CAB8811)Western blot analysis of extracts of various cell lines, using E2F3 antibody (CAB8811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

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