E2F1 Rabbit Monoclonal Antibody (CAB19579)
- SKU:
- CAB19579
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
Product Name: | E2F1 Rabbit Monoclonal Antibody |
SKU: | CAB19579 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 338-437 of human E2F1 (Q01094). |
Sequence: | PPSS LTTD PSQS LLSL EQEP LLSR MGSL RAPV DEDR LSPL VAAD SLLE HVRE DFSG LLPE EFIS LSPP HEAL DYHF GLEE GEGI RDLF DCDF GDLT PLDF |
Tested Applications: | WB IP ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | RBP3; E2F-1; RBAP1; RBBP3; E2F1 |
Positive Sample: | U-87MG,HT-29,HeLa,A-431,Mouse testis,Mouse brain,Rat thymus,Rat spleen |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 47kDa |
Observed MW: | 70kDa |
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Purification Method: | Affinity purification |
Gene ID: | 1869 |
Clone Number: | ARC0058 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |