The E2A/TCF3 Monoclonal Antibody (ABM51049), designed by Assay Genie, it is specifically engineered for research focusing on the E2A protein, also known as TCF3, which plays a critical role in gene regulation and transcription. This antibody, produced through monoclonal technology, offers high specificity and sensitivity for detecting E2A/TCF3 in human samples, making it a powerful tool for studies in molecular biology and cancer research.The E2A protein is a transcription factor that regulates the expression of genes involved in cell differentiation and development, making it a key player in various cellular processes. Its dysregulation has been linked to several types of cancer, making it a potential target for therapeutic interventions.
By utilizing the E2A/TCF3 Monoclonal Antibody, researchers can effectively study the expression and function of this protein in different cell types, aiding in the advancement of our understanding of its role in disease development.The validation of this antibody for Western blot applications ensures accurate and reliable results, enabling researchers to confidently analyze the expression levels of E2A/TCF3 in their experimental samples. With its high reactivity and specificity, the E2A/TCF3 Monoclonal Antibody is a valuable tool for scientists looking to unravel the complexities of gene regulation and its implications in cancer biology.
Product Name:
E2A/TCF3 Monoclonal Antibody
SKU:
CAB23946
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 379-525 of human E2A/TCF3（NP_003191.1）.
This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.
Purification Method:
Affinity purification
Gene ID:
6929
Clone Number:
ARC61947
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of Mouse ovary, using E2A/TCF3 Rabbit mAb (CAB23946) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
