The E2A Polyclonal Antibody (APC09541) is a valuable tool for researchers studying the E2A protein, a transcription factor that plays a critical role in various cellular processes. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in immunohistochemistry and immunofluorescence applications.E2A is known to be involved in the regulation of gene expression, cell differentiation, and development, making it a key target for investigation in fields such as developmental biology and cancer research.
The E2A Polyclonal Antibody binds to the E2A protein, enabling researchers to visualize and analyze its expression in different cell types and tissues.By studying the role of E2A in cellular processes, researchers can gain valuable insights into mechanisms underlying normal development and disease progression. The E2A Polyclonal Antibody provides a reliable tool for investigating the function of E2A and its potential as a therapeutic target in various pathological conditions.
Product Name:
E2A Polyclonal Antibody
SKU:
CAB22543
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 450-525 of human E2A. (P15923).
This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.
Purification Method:
Affinity purification
Gene ID:
6929
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using E2A Rabbit pAb (CAB22543) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
