The DYNC2LI1 Polyclonal Antibody (CAB4752) is a research tool designed for studying the DYNC2LI1 protein, which is involved in intracellular transport processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the DYNC2LI1 protein, this antibody enables researchers to detect and analyze this key molecule in various cell types, making it an essential tool for studies in cell biology and molecular transport mechanisms.DYNC2LI1 is a crucial component of the dynein-2 motor complex, which plays a fundamental role in retrograde intraflagellar transport and ciliary function.
Dysregulation of DYNC2LI1 has been associated with ciliary dysfunction and ciliopathies, making it a significant target for research in the fields of developmental biology and genetic disorders. Understanding the function and regulation of DYNC2LI1 is essential for uncovering the molecular mechanisms underlying ciliary-related diseases and developing targeted therapeutic interventions.
Product Name:
DYNC2LI1 Rabbit Polyclonal Antibody
SKU:
CAB4752
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human DYNC2LI1 (NP_001180393.1).
This gene encodes a protein that is a component of the dynein-2 microtubule motor protein complex that plays a role in the retrograde transport of cargo in primary cilia via the intraflagellar transport system. This gene is ubiquitously expressed and its protein, which localizes to the axoneme and Golgi apparatus, interacts directly with the cytoplasmic dynein 2 heavy chain 1 protein to form part of the multi-protein dynein-2 complex. Mutations in this gene produce defects in the dynein-2 complex which result in several types of ciliopathy including short-rib thoracic dysplasia 15 with polydactyly (SRTD15). Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
51626
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DYNC2LI1 antibody (CAB4752) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
