The Dynactin 1 (DCTN1) Polyclonal Antibody (CAB18314) is crafted for investigations involving DCTN1, a key protein involved in intracellular transport and cytoskeletal organization. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been rigorously validated for use in Western blotting applications. By specifically binding to the DCTN1 protein, this antibody enables accurate detection and analysis across various cell types, making it an invaluable tool for studies in cell biology and neurodegenerative diseases.
DCTN1, also known as p150 Glued, plays a crucial role in linking vesicles to cytoplasmic dynein during retrograde transport along microtubules. Dysfunction or dysregulation of DCTN1 has been implicated in neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) and Charcot-Marie-Tooth disease (CMT). Understanding the function and regulation of DCTN1 is essential for unraveling the pathophysiology of these conditions and may offer insights into potential therapeutic targets for treating these debilitating diseases.
Product Name:
DYNC1H1 Polyclonal Antibody
SKU:
CAB18314
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DYNC1H1 (NP_001367.2).
Dyneins are a group of microtubule-activated ATPases that function as molecular motors. They are divided into two subgroups of axonemal and cytoplasmic dyneins. The cytoplasmic dyneins function in intracellular motility, including retrograde axonal transport, protein sorting, organelle movement, and spindle dynamics. Molecules of conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains.This gene encodes a member of the cytoplasmic dynein heavy chain family.
Purification Method:
Affinity purification
Gene ID:
1778
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of 293F, using DYNC1H1 Rabbit pAb (CAB18314) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
