DUT Rabbit Polyclonal Antibody (CAB2901)
- SKU:
- CAB2901
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Product Name: | DUT Rabbit Polyclonal Antibody |
SKU: | CAB2901 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1). |
Sequence: | PAPG PETP AISP SKRA RPAE VGGM QLRF ARLS EHAT APTR GSAR AAGY DLYS AYDY TIPP MEKA VVKT DIQI ALPS GCYG RVAP RSGL AAKH FIDV GAGV IDED YRGN VGVV LFNF GKEK FEVK KGDR IAQL ICER IFYP EIEE VQAL DDTE RGSG GFGS TGKN |
Tested Applications: | WB IHC-P IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:100 - 1:200 |
Synonyms: | BMFDMS; dUTPase; DUT |
Positive Sample: | LO2,Jurkat,HeLa |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion, Nucleus. |
Calculated MW: | 27kDa |
Observed MW: | 24kDa |
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Purification Method: | Affinity purification |
Gene ID: | 1854 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |