The DUT Polyclonal Antibody (CAB2901) is a valuable tool for researchers studying DUT, also known as deoxyuridine 5'-triphosphate nucleotidohydrolase. DUT is an essential enzyme involved in DNA metabolism, specifically in the prevention of uracil incorporation into DNA. This antibody is produced in rabbits and is highly specific to human samples, making it ideal for Western blot applications. By binding to the DUT protein, researchers are able to detect and analyze its expression in various cell types, providing insights into its role in DNA repair and maintenance.
DUT dysfunction has been linked to a variety of diseases, including cancer and neurological disorders, making it a promising target for drug development and therapeutic interventions. By studying the activity of DUT using this antibody, researchers can gain a better understanding of its function and potential implications for human health.
Product Name:
DUT Rabbit Polyclonal Antibody
SKU:
CAB2901
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1).
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Purification Method:
Affinity purification
Gene ID:
1854
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DUT Rabbit pAb (CAB2901) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
