DUT Monoclonal Antibody (CAB23563)
- SKU:
- CAB23563
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
Product Name: | DUT Monoclonal Antibody |
SKU: | CAB23563 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-252 of human DUT (NP_001020419.1). |
Sequence: | IALP SGCY GRVA PRSG LAAK HFID VGAG VIDE DYRG NVGV VLFN FGKE KFEV KKGD RIAQ LICE RIFY PEIE EVQA LDDT ERGS GGFG STGK N |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 |
Synonyms: | BMFDMS; dUTPase; DUT |
Positive Sample: | Jurkat |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion, Nucleus. |
Calculated MW: | 27kDa |
Observed MW: | 23kDa |
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Purification Method: | Affinity purification |
Gene ID: | 1854 |
Clone Number: | ARC3127 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |