The DUT Monoclonal Antibody is a cutting-edge tool for researchers studying the enzyme dUTPase, which plays a critical role in DNA replication and repair processes. This monoclonal antibody, developed using advanced technologies, is highly specific and sensitive for detecting dUTPase in various biological samples.DUT, or deoxyuridine triphosphatase, is essential for maintaining genomic stability by preventing the incorporation of uracil into DNA. Dysregulation of dUTPase activity has been linked to genomic instability and diseases such as cancer. Therefore, understanding the function and regulation of dUTPase is vital for advancing research in oncology and DNA repair mechanisms.
The DUT Monoclonal Antibody is validated for use in techniques such as immunofluorescence, immunohistochemistry, and Western blotting. Its high affinity and specificity make it a valuable tool for investigating the expression and localization of dUTPase in different cell types and tissues. Researchers can rely on this antibody to explore the role of dUTPase in DNA damage response pathways and its potential as a therapeutic target in cancer treatment strategies.
Product Name:
DUT Monoclonal Antibody
SKU:
CAB23563
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-252 of human DUT (NP_001020419.1).
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Purification Method:
Affinity purification
Gene ID:
1854
Clone Number:
ARC3127
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using DUT Rabbit mAb (CAB23563) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
