The DUSP10 Polyclonal Antibody (CAB8748) is a powerful tool for research involving DUSP10, a dual-specificity phosphatase that plays a crucial role in MAPK signaling pathways. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the DUSP10 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in signal transduction and cell signaling pathways.DUSP10, also known as MKP-5, is involved in the dephosphorylation of MAP kinases, including ERK, JNK, and p38, thereby regulating their activity and downstream signaling cascades. Its role in controlling cell growth, differentiation, and apoptosis makes it a key player in various physiological and pathological processes.
Research on DUSP10 is particularly valuable in understanding diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, where dysregulation of MAPK signaling is implicated.By using the DUSP10 Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of DUSP10, paving the way for the development of novel therapeutic strategies targeting MAPK signaling pathways in various disease settings. Its versatility and specificity make it an essential tool for advancing scientific knowledge in the fields of molecular biology, biochemistry, and drug discovery.
Product Name:
DUSP10 Rabbit Polyclonal Antibody
SKU:
CAB8748
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DUSP10 (NP_009138.1).
Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
11221
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of BT-474 cells, using DUSP10 antibody (CAB8748) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
