The DUSP1/DUSP4 Antibody (PACO07149) is a polyclonal antibody designed for research involving the dual-specificity phosphatases DUSP1 and DUSP4. These phosphatases play a critical role in the regulation of signaling pathways involved in cell growth, differentiation, and immune responses. The antibody, raised in rabbits, is highly specific and reactive with human samples, making it a valuable tool for studying the function and activity of DUSP1 and DUSP4 in various biological processes.This antibody is validated for use in Western blot applications, allowing for the detection and analysis of DUSP1 and DUSP4 proteins in different cell types and tissues.
Its high specificity ensures accurate results and reliable data for researchers in the fields of immunology, cancer biology, and cell signaling pathways.Understanding the role of DUSP1 and DUSP4 in cell signaling and immune regulation is crucial for developing targeted therapies for diseases such as cancer, inflammatory disorders, and autoimmune conditions. The DUSP1/DUSP4 Antibody (PACO07149) provides researchers with a powerful tool to unravel the intricacies of these phosphatases and their potential as therapeutic targets in various disease settings.
Antibody Name:
DUSP1/DUSP4 Antibody (PACO07149)
Antibody SKU:
PACO07149
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
ELISA:1:20000, WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from human MKP-1/2 around the non-phosphorylation site of S296/318.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of Hela cells using MKP-1/2 Polyclonal Antibody.
Western blot analysis of 293T lysis using MKP-1/2 antibody.
Western Blot analysis of Jurkat cells using MKP-1/2 Polyclonal Antibody.
Synonyms:
DUSP1; CL100; MKP1; PTPN10; VH1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100; DUSP4;
UniProt Protein Function:
MKP-1: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP1 specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation. It is induced in human skin fibroblasts by oxidative/heat stress and growth factors. Contains 1 rhodanese domain.
UniProt Protein Details:
Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis; EC 3.1.3.16
Chromosomal Location of Human Ortholog: 5q34
Cellular Component: cytoplasm; nucleus
Molecular Function:protein tyrosine/threonine phosphatase activity; protein binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity
Biological Process: negative regulation of MAP kinase activity; response to light stimulus; response to retinoic acid; response to cAMP; negative regulation of MAPKKK cascade; positive regulation of apoptosis; response to glucocorticoid stimulus; negative regulation of meiotic cell cycle; response to testosterone stimulus; protein amino acid dephosphorylation; response to estradiol stimulus; cellular response to hormone stimulus; regulation of apoptosis; response to hydrogen peroxide; endoderm formation; response to oxidative stress; response to calcium ion; negative regulation of apoptosis; inactivation of MAPK activity
NCBI Summary:
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]
