The DTL Polyclonal Antibody (CAB12150) is a valuable tool for researchers studying the DTL protein, which is crucial for cell cycle regulation and DNA replication. This antibody, produced in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples. By specifically targeting the DTL protein, this antibody allows for the detection and analysis of DTL in various cell types, making it ideal for studies in cell biology, cancer research, and DNA replication mechanisms.
The DTL protein, also known as Cdt2, is involved in the degradation of key cell cycle regulators and plays a critical role in maintaining genomic stability. Dysregulation of DTL has been implicated in various diseases, including cancer, making it a promising target for therapeutic interventions. Understanding the function of DTL is essential for unraveling its role in disease development and progression, paving the way for potential new treatments targeting this pathway.
Product Name:
DTL Rabbit Polyclonal Antibody
SKU:
CAB12150
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 541-730 of human DTL (NP_057532.3).
Contributes to ubiquitin-protein transferase activity. Involved in several processes, including protein ubiquitination; regulation of G2/M transition of mitotic cell cycle; and translesion synthesis. Located in centrosome; cytosol; and nuclear lumen. Part of Cul4A-RING E3 ubiquitin ligase complex and Cul4B-RING E3 ubiquitin ligase complex.
Purification Method:
Affinity purification
Gene ID:
51514
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DTL Rabbit pAb (CAB12150) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
