Anti-DSPP Antibody (CAB8413)

Antibody Name:	Anti-DSPP Antibody
Antibody SKU:	CAB8413
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-146 of human DSPP (NP_055023.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HL-60

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-146 of human DSPP (NP_055023.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IPVP QSKP LERH VEKS MNLH LLAR SNVS VQDE LNAS GTIK ESGV LVHE GDRG RQEN TQDG HKGE GNGS KWAE VGGK SFST YSTL ANEE GNIE GWNG DTGK AETY GHDG IHGK EENI TANG IQGQ VSII DNA
Gene ID:	1834
Uniprot:	Q9NZW4
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	131kDa
Observed MW:	110kDa

Synonyms:

DSPP, DFNA39, DGI1, DMP3, DPP, DSP

Background:

This gene encodes a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of proteins. The encoded preproprotein is secreted by odontoblasts and proteolytically processed to generate two principal proteins of the dentin extracellular matrix of the tooth, dentin sialoprotein and dentin phosphoprotein. These two protein products may play distinct but related roles in dentin mineralization. Mutations in this gene are associated with dentinogenesis imperfecta and dentin dysplasia. This gene is present in a gene cluster on chromosome 4. Allelic differences due to repeat polymorphisms have been found for this gene.

UniProt Protein Function:	DSPP: DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals. Defects in DSPP are the cause of deafness autosomal dominant type 39 with dentinogenesis imperfecta 1 (DFNA39/DGI1). Affected individuals present DGI1 associated with early onset progressive sensorineural high-frequency hearing loss. Defects in DSPP are the cause of dentinogenesis imperfecta type 1 (DGI1); also known as dentinogenesis imperfecta Shields type 2 (DGI2). DGI1 is an autosomal dominant disorder in which both the primary and the permanent teeth are affected. It occurs with an incidence of 1:8000 live births. The teeth are amber and opalescent, the pulp chamber being obliterated by abnormal dentin. The enamel, although unaffected, tends to fracture, which makes dentin undergo rapid attrition, leading to shortening of the teeth. Defects in DSPP are a cause of dentinogenesis imperfecta Shields type 3 (DGI3). Patients with DGI3 do not have stigmata of osteogenesis imperfecta. The finding that a single defects in the DSPP gene causes both phenotypic patterns of DGI2 and DGI3 strongly supports the conclusion that these two disorders are not separate diseases but rather the phenotypic variation of a single genetic defect. Defects in DSPP are the cause of dentin dysplasia type 2 (DTDP2); also known as dentin dysplasia Shields type 2. DTDP2 is an autosomal dominant disorder in which mineralization of the dentine of the primary teeth is abnormal. On the basis of the phenotypic overlap between, and shared chromosomal location with DGI2 it has been proposed that DTDP2 and DGI2 are allelic. From the results of recent studies, it is clear that different types of mutations in DSPP lead to the two different phenotypes.
UniProt Protein Details:	Protein type:Extracellular matrix; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 4q21.3 Cellular Component: cytoplasm; extracellular region Molecular Function:calcium ion binding; collagen binding; extracellular matrix structural constituent Biological Process: extracellular matrix organization and biogenesis; multicellular organismal development; ossification; skeletal development Disease: Deafness, Autosomal Dominant 39, With Dentinogenesis Imperfecta 1; Dentin Dysplasia, Type Ii; Dentinogenesis Imperfecta 1; Dentinogenesis Imperfecta, Shields Type Iii
NCBI Summary:	This gene encodes a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of proteins. The encoded preproprotein is secreted by odontoblasts and proteolytically processed to generate two principal proteins of the dentin extracellular matrix of the tooth, dentin sialoprotein and dentin phosphoprotein. These two protein products may play distinct but related roles in dentin mineralization. Mutations in this gene are associated with dentinogenesis imperfecta and dentin dysplasia. This gene is present in a gene cluster on chromosome 4. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jan 2016]
UniProt Code:	Q9NZW4
NCBI GenInfo Identifier:	89001107
NCBI Gene ID:	1834
NCBI Accession:
UniProt Secondary Accession:	Q9NZW4,O95815, A8MUI0,
UniProt Related Accession:	Q9NZW4
Molecular Weight:
NCBI Full Name:	dentin sialophosphoprotein preproprotein
NCBI Synonym Full Names:	dentin sialophosphoprotein
NCBI Official Symbol:	DSPP
NCBI Official Synonym Symbols:	DPP; DSP; DGI1; DMP3; DFNA39
NCBI Protein Information:	dentin sialophosphoprotein
UniProt Protein Name:	Dentin sialophosphoprotein
UniProt Synonym Protein Names:	Dentin phosphoproteinAlternative name(s):Dentin phosphophoryn; DPP
Protein Family:	Dentin sialophosphoprotein
UniProt Gene Name:	DSPP
UniProt Entry Name:	DSPP_HUMAN

	Western blot analysis of extracts of various cell lines, using DSPP antibody (CAB8413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of H9C2 cells using DSPP Rabbit pAb (CAB8413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using DSPP Rabbit pAb (CAB8413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using DSPP Rabbit pAb (CAB8413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ELISA Kits

Human Dentin sialophosphoprotein (DSPP) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)