DRM (Doramectin) ELISA Kit (FSES0054)- High Sensitivity

DRM (Doramectin) ELISA Kit (FSES0054)

SKU:

FSES0054

Product Type:

ELISA Kit

Applications:

ELISA

Research Area:

Food Science

Description

Description

DRM (Doramectin) ELISA Kit

Kit Name:

DRM (Doramectin) ELISA Kit

Kit Size:

96 Assays

Test Principle:

This kit uses Competitive-ELISA as the method. It can detect Doramectin (DRM) in samples, such as raw milk, finished milk, yogurt, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, DRM in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-DRM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of DRM. The concentration of DRM in the samples can be calculated by comparing the OD of the samples to the standard curve.

Sensitivity:

0.1 ppb (ng/mL)

Reaction Mode:

25°C, 30 min~30min~15min

Detection Limit:

Raw milk, Finished milk, Yogurt --- 1 ppb

Cross Reactivity:

Doramectin	100%
Abamectin	300%
Ivermectin	227%
Eprinomectin	300%

Sample Recovery Rate:

100%±20%.

Kits Components:

Item	Specifications
ELISA Microtiter plate	96 wells
Standard Liquid	1 mL each< (0 ppb, 0.1 ppb, 0.2 ppb, 0.4 ppb, 0.8 ppb, 1.6 ppb)
Antibody Working Solution	7 mL
HRP Conjugate	12 mL
Milk Precipitant	10 mL
Sample Diluent A	20 mL
Sample Diluent B	20 mL
Substrate Reagent A	6 mL
Substrate Reagent B	6 mL
Stop Solution	6 mL
20×Concentrated Wash Buffer	25 mL
Plate Sealer	3 pieces
Sealed Bag	1 piece
Manual	1 copy

Instruments:

Microplate reader, Oscillators, Centrifuge.

Micropipette:

Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).

Reagents:

Methanol.

Storage And Expiry Date:

Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.