The DPY19L3 Antibody (PAC058212) is a highly specific and sensitive tool for researchers studying the DPY19L3 protein. This polyclonal antibody, raised in rabbits, is optimized for use in various molecular biology applications, including Western blot analysis and immunofluorescence assays.DPY19L3 is a critical protein involved in sperm maturation and male fertility. Research has shown that mutations in the DPY19L3 gene can lead to male infertility due to abnormal sperm development. By targeting and detecting DPY19L3 in samples, researchers can gain valuable insights into the molecular mechanisms underlying male fertility and reproductive health.
This antibody is ideal for scientists investigating reproductive biology, infertility, and genetic disorders related to male fertility. Its high specificity and sensitivity make it a valuable tool for understanding the function and regulation of DPY19L3 in cells and tissues. Harnessing the power of the DPY19L3 Antibody can lead to advancements in fertility treatments and personalized medicine approaches.
Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate. All lanes: DPY19L3 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84, 63 kDa. Observed band size: 84 kDa.
Immunofluorescence staining of HepG2 cells with PACO58212 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Probable C-mannosyltransferase that mediates C-mannosylation of tryptophan residues on target proteins.
DPY19L3: Belongs to the dpy-19 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Membrane protein, multi-passChromosomal Location of Human Ortholog: 19q13.11Cellular Component: integral to membrane; nuclear inner membraneMolecular Function: mannosyltransferase activityBiological Process: protein amino acid C-linked glycosylation via 2'-alpha-mannosyl-L-tryptophan
