The DPY19L2 Polyclonal Antibody (PACO60164) is a valuable tool for research involving DPY19L2, a protein involved in spermatogenesis and male fertility. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the DPY19L2 protein, this antibody allows for precise detection and analysis in various cell types, making it an essential reagent for studies in reproductive biology and infertility research.DPY19L2 is known to play a critical role in sperm development and function, making it a key player in male fertility.
Understanding the function and regulation of DPY19L2 is crucial for uncovering the mechanisms underlying infertility and developing potential treatments or interventions for individuals facing fertility challenges. The DPY19L2 Polyclonal Antibody provides researchers with a powerful tool to further explore the role of DPY19L2 in reproduction and potentially advance our understanding of male infertility.
Antibody Name:
DPY19L2 Antibody (PACO60164)
Antibody SKU:
PACO60164
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Probable C-mannosyltransferase DPY19L2 protein (599-749AA)
Immunofluorescence staining of A549 cells with PACO60164 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Probable C-mannosyltransferase that mediates C-mannosylation of tryptophan residues on target proteins (By similarity). Required during spermatogenesis for sperm head elongation and acrosome formation.
DPY19L2: Required during spermatogenesis for sperm head elongation and acrosome formation. Defects in DPY19L2 are the cause of spermatogenic failure type 9 (SPGF9). An infertility disorder caused by spermatogenesis defects. The most prominent feature is the malformation of the acrosome, which can be totally absent in most severe cases. Additional features are an abnormal nuclear shape and abnormal arrangement of the mitochondria of the spermatozoon. Deletions in DPY19L2 are probably the major cause of SPGF9. Belongs to the dpy-19 family.Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 12q14.2Cellular Component: integral to membrane; nuclear inner membrane; nucleusMolecular Function: mannosyltransferase activityBiological Process: multicellular organismal development; protein amino acid C-linked glycosylation via 2'-alpha-mannosyl-L-tryptophan; spermatid developmentDisease: Spermatogenic Failure 9
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the dpy-19 family. It is highly expressed in testis, and is required for sperm head elongation and acrosome formation during spermatogenesis. Mutations in this gene are associated with an infertility disorder, spermatogenic failure type 9 (SPGF9). [provided by RefSeq, Dec 2011]
