The DPP10 Polyclonal Antibody (CAB16563) is a valuable tool for research involving the DPP10 protein, a member of the dipeptidyl peptidase family implicated in various physiological processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the DPP10 protein, this antibody enables researchers to detect and analyze DPP10 expression in different cell types, making it an ideal choice for studies in the fields of biochemistry and molecular biology.
DPP10 is known to play a critical role in ion channel regulation, specifically affecting potassium channels in neurons and pancreatic beta cells. Its involvement in regulating membrane potential and neurotransmitter release makes it a promising target for research into neurological disorders and diabetes. Understanding the function of DPP10 is essential for developing potential therapies that target ion channel activity and its implications in disease pathology.
Product Name:
DPP10 Rabbit Polyclonal Antibody
SKU:
CAB16563
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human DPP10 (NP_001308842.1).
Sequence:
GYIA SMIL KSDE KLFK CGSV VAPI TDLK LYAS AFSE RYLG MPSK EEST YQAA SVLH NVHG LKEE NILI IHGT ADTK VHFQ HSAE LIKH LIKA GVNY TMQV Y
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
DPL2; DPPY; DPRP3; DPRP-3; DPP10
Positive Sample:
U-251MG
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type II membrane protein.
Calculated MW:
91kDa
Observed MW:
110kDa
This gene encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method:
Affinity purification
Gene ID:
57628
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-251MG cells, using DPP10 Rabbit pAb (CAB16563) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
