The DPM1 Polyclonal Antibody (CAB7757) is a valuable tool for researchers studying DPM1, a key enzyme involved in the biosynthesis of dolichol phosphate-mannose. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. It specifically targets the DPM1 protein, allowing for precise detection and analysis in a variety of cell types, making it ideal for investigations in biochemistry and glycobiology.DPM1 plays a crucial role in the synthesis of dolichol phosphate-mannose, a molecule essential for the N-linked glycosylation process.
Dysfunction of this enzyme has been linked to various disorders, including congenital disorders of glycosylation. Research on DPM1 is essential for unraveling the mechanisms underlying these diseases and developing potential therapeutic interventions aimed at restoring normal glycosylation patterns.By utilizing the DPM1 Polyclonal Antibody (CAB7757), researchers can gain valuable insights into the function and regulation of DPM1, paving the way for advancements in the understanding and treatment of glycosylation-related disorders.
Product Name:
DPM1 Rabbit Polyclonal Antibody
SKU:
CAB7757
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DPM1 (NP_003850.1).
Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8813
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DPM1 antibody (CAB7757) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
