The DPH6 Polyclonal Antibody (PACO36926) is a valuable tool for research involving the DPH6 protein, which is an enzyme involved in the biosynthesis of diphthamide, a modified histidine residue found in eukaryotic elongation factor 2 (eEF2). This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding to the DPH6 protein, this antibody allows for the detection and analysis of DPH6 levels in a variety of cell types, making it an ideal choice for studies in molecular biology and cancer research.DPH6 is a key player in the process of diphthamide biosynthesis, which is essential for the proper functioning of eEF2 in protein translation.
Aberrant expression or activity of DPH6 has been implicated in various diseases, including cancer, making it a promising target for therapeutic interventions. Research on DPH6 can provide valuable insights into the molecular mechanisms underlying disease pathogenesis and may lead to the development of novel treatment strategies. By utilizing the DPH6 Polyclonal Antibody, researchers can delve deeper into the biology of DPH6 and its potential role in disease progression.
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO36926 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO36926 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor (EEF2) to diphthamide.
DPH6: Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor (EEF2) to diphthamide. Belongs to the Diphthine--ammonia ligase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 6.3.1.14Chromosomal Location of Human Ortholog: 15q14Cellular Component: cytosol; nucleolus; nucleusMolecular Function: ATP binding; diphthine-ammonia ligase activityBiological Process: cellular protein metabolic process; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; post-translational protein modification; regulation of protein stability
