The DPH3 Polyclonal Antibody (PAC038146) is a valuable tool for researchers studying DPH3, a protein involved in protein synthesis and RNA processing. This antibody, generated in rabbits, has high reactivity with human samples and is suitable for use in Western blot applications. By binding to the DPH3 protein, this antibody allows for the detection and analysis of DPH3 in various cell types, making it a versatile tool for studies in molecular biology and genetics.DPH3 is an essential component of the diphthamide biosynthesis pathway, which is involved in the modification of translation elongation factor 2 (eEF2) and plays a crucial role in protein synthesis.
Understanding the function of DPH3 is important for unraveling the mechanisms behind protein synthesis and RNA processing. Researchers studying cellular processes such as translation regulation and ribosome biogenesis will benefit from using this antibody in their experiments.Overall, the DPH3 Polyclonal Antibody is a reliable reagent for investigating the role of DPH3 in various biological processes. Its specificity and sensitivity make it an ideal choice for researchers conducting studies in molecular biology, genetics, and related fields.
Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2 (EEF2) and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta).
Synonyms:
DPH3 homolog (CSL-type zinc finger-containing protein 2) (DelGEF-interacting protein 1) (DelGIP1), DPH3, DESR1 ZCSL2
UniProt Protein Function:
DPH3: Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2 and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A. Belongs to the DPH3 family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 3p25.1Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosolMolecular Function: protein binding; metal ion bindingBiological Process: negative regulation of protein secretion; cellular protein metabolic process; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; positive regulation of binding; post-translational protein modification
UniProt Protein Details:
NCBI Summary:
This gene encodes a CSL zinc finger-containing protein that is required for dipthamide biosynthesis. The encoded protein is necessary for the initial step in the modification of a histidine residue in elongation factor-2 to diphthamide. This modified residue is a target for ADP ribosylation by the bacterial toxins diphtheria toxin and Pseudomonas exotoxin A. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Feb 2009]
