The Doublecortin Monoclonal Antibody offered by Assay Genie (ABM1012) is a valuable tool for researchers studying the Doublecortin protein, which plays a key role in neuronal migration and differentiation in the developing brain. This antibody, produced using recombinant DNA technology, has been rigorously tested and validated for use in immunofluorescence and immunohistochemistry applications.Doublecortin is a protein that is specifically expressed in neural precursor cells and immature neurons, making it a useful marker for studying neurogenesis and neuronal development.
By using the Doublecortin Monoclonal Antibody, researchers can accurately detect and visualize Doublecortin in tissue samples, allowing for detailed analysis of neurogenesis processes.This antibody is highly specific to Doublecortin and shows minimal cross-reactivity with other proteins, ensuring reliable and accurate results in experiments. With its high sensitivity and specificity, the Doublecortin Monoclonal Antibody is an essential tool for researchers investigating neurodevelopmental disorders, brain injuries, and neurodegenerative diseases.
Product Name:
Doublecortin Monoclonal Antibody
SKU:
CAB23501
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant protein of human Doublecortin.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:2000 - 1:8000
Synonyms:
DC; DBCN; LISX; SCLH; XLIS; Doublecortin
Positive Sample:
SH-SY5Y,Mouse brain,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Cell projection, Cytoplasm.
Calculated MW:
41kDa
Observed MW:
41kDa
This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1641
Clone Number:
ARC57902
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Doublecortin Rabbit mAb (CAB23501) at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
