The DOT1L Polyclonal Antibody (CAB7619) is a valuable tool for researchers studying the DOT1L protein, an enzyme involved in histone methylation and gene regulation. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in a variety of applications, including Western blot and immunohistochemistry.DOT1L is a key player in epigenetic regulation, influencing gene expression and cellular function. Dysregulation of DOT1L has been implicated in various diseases, including leukemia and heart failure, making it an important target for therapeutic research.
By using the DOT1L Polyclonal Antibody, researchers can accurately detect and quantify levels of DOT1L in different cell types and tissues, aiding in the study of its role in health and disease.Overall, the DOT1L Polyclonal Antibody is a crucial tool for exploring the functions of DOT1L and its potential implications in various pathological conditions, providing valuable insights for drug discovery and therapeutic development.
Product Name:
Dot1L/KMT4 Rabbit Polyclonal Antibody
SKU:
CAB7619
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-500 of human Dot1L/KMT4 (NP_115871.1).
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes.
Purification Method:
Affinity purification
Gene ID:
84444
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Dot1L/KMT4 antibody (CAB7619) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
