The DOT1L Polyclonal Antibody (CAB11285) is a valuable tool for researchers studying the DOT1L protein, which plays a key role in gene regulation and is implicated in diseases such as leukemia. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the DOT1L protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in cancer research and epigenetics.DOT1L, also known as disruptor of telomeric silencing 1-like, is a histone methyltransferase that regulates gene expression through the methylation of histone H3 at lysine 79.
Dysregulation of DOT1L has been associated with various types of cancer, making it a promising target for therapeutic interventions. Research on DOT1L is crucial for understanding its role in gene regulation and identifying potential treatment strategies for cancer patients.By using the DOT1L Polyclonal Antibody, researchers can gain valuable insights into the function of DOT1L in normal and diseased cells, paving the way for innovative approaches to cancer therapy and drug development. This antibody is a vital tool for investigating the mechanisms underlying DOT1L-mediated gene regulation and its implications for cancer biology.
Product Name:
Dot1L/KMT4 Rabbit Polyclonal Antibody
SKU:
CAB11285
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Dot1L/KMT4 (NP_115871.1).
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes.
Purification Method:
Affinity purification
Gene ID:
84444
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat liver, using Dot1L/KMT4 Rabbit pAb (CAB11285) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
