DOK3 Antibody (PACO14350)
- SKU:
- PACO14350
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
DOK3 Antibody (PACO14350)
The DOK3 Polyclonal Antibody (PAC014350) is a valuable tool for researchers studying DOK3, a protein involved in immune cell signaling pathways. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the DOK3 protein, this antibody allows for the detection and analysis of DOK3 in a variety of cell types, making it a versatile tool for immunology and cancer research studies.DOK3, a member of the downstream of tyrosine kinase (DOK) family, plays a crucial role in regulating immune cell activation and function. It is particularly important in the context of immune responses to pathogens, inflammation, and autoimmunity.
Research on DOK3 is key to understanding the complexities of immune system regulation and developing targeted therapies for immune-related disorders.Overall, the DOK3 Polyclonal Antibody is a valuable resource for researchers investigating immune signaling pathways and potential therapeutic targets in conditions such as cancer, autoimmune diseases, and inflammatory disorders. Its high specificity and reliability in Western blot applications make it an essential tool for advancing our knowledge of immune system function and dysfunction.
| Antibody Name: | DOK3 Antibody (PACO14350) |
| Antibody SKU: | PACO14350 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
| Species Reactivity: | Human |
| Immunogen: | Fusion protein of human DOK3 |
| Form: | Liquid |
| Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Purification Method: | Antigen affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Human notum skin cancer tissue, Primary antibody: PACO14350(DOK3 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds. |
 | The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO14350(DOK3 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200). |
| Background: | DOK3 gene maps to chromosome 5q35.3. Dok3 was tyrosine phosphorylated by Src family members Lck, Fyn, and Lyn. Immunoprecipitation studies showed that Dok3 bound inhibitors SHIP and Csk but did not bind RasGAP. Dok3 binding to SHIP occurred via the SH2 domain. Dok3 also bound Csk via the Csk SH2 domain with possible involvement of the Csk SH3 domain as well.DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function |
| Synonyms: | docking protein 3 |
| UniProt Protein Function: | DOK3: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with SHIP. May modulate Abl function. Upon tyrosine phosphorylation, Dok3 interacts with CSK and SHIP via their SH2 domains. Both pY381 and pY398 are required for interaction with SHIP. Only pY381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Does not interact with RasGAP. Four isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 5q35.3 Molecular Function:protein binding |
| UniProt Code: | Q7L591 |
| NCBI GenInfo Identifier: | 84029599 |
| NCBI Gene ID: | 79930 |
| NCBI Accession: | Q7L591.2 |
| UniProt Secondary Accession: | Q7L591,Q8N864, Q9BQB3, Q9H666, E9PAT0, H7BXS0, |
| UniProt Related Accession: | Q7L591 |
| Molecular Weight: | 24,737 Da |
| NCBI Full Name: | Docking protein 3 |
| NCBI Synonym Full Names: | docking protein 3 |
| NCBI Official Symbol: | DOK3Â Â |
| NCBI Official Synonym Symbols: | DOKLÂ Â |
| NCBI Protein Information: | docking protein 3 |
| UniProt Protein Name: | Docking protein 3 |
| UniProt Synonym Protein Names: | Downstream of tyrosine kinase 3 |
| Protein Family: | Docking protein |
| UniProt Gene Name: | DOK3Â Â |
| UniProt Entry Name: | DOK3_HUMAN |
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