The DOHH Antibody (PAC060376) is a highly specific polyclonal antibody designed for research involving DOHH, a key enzyme involved in post-translational modification of proteins. This antibody, produced in rabbits, is suitable for use in various applications including Western blot and immunohistochemistry.DOHH plays a crucial role in the hydroxylation process of asparaginyl residues of translation elongation factor eEF1A, which is essential for protein synthesis. Dysregulation of DOHH has been linked to various diseases, including cancer and neurodegenerative disorders.
Therefore, studying DOHH expression and activity is important for understanding its role in disease development and progression.This antibody specifically binds to DOHH, allowing for accurate detection and analysis of DOHH expression levels in various cell types and tissues. With its high reactivity and specificity, the DOHH Antibody (PAC060376) is an invaluable tool for researchers studying protein modification processes and their implications in disease pathology.
Antibody Name:
DOHH Antibody (PACO60376)
Antibody SKU:
PACO60376
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Deoxyhypusine hydroxylase protein (79-159AA)
Immunofluorescence staining of PC-3 cells with PACO60376 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
DOHH: Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)- L-lysine intermediate to form hypusine, an essential post- translational modification only found in mature eIF-5A factor. Belongs to the deoxyhypusine hydroxylase family.Protein type: Oxidoreductase; EC 1.14.99.29Chromosomal Location of Human Ortholog: 19p13.3Cellular Component: cytosolMolecular Function: protein binding; metal ion binding; deoxyhypusine monooxygenase activityBiological Process: cellular protein metabolic process; hypusine biosynthetic process from peptidyl-lysine; post-translational protein modification
UniProt Protein Details:
NCBI Summary:
This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]
