DOCK1 Antibody (PACO54486)
- SKU:
- PACO54486
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
DOCK1 Antibody (PACO54486)
The Dock1 Polyclonal Antibody (PAC054486) is a valuable tool for researchers studying the Dock1 protein, a key regulator of cell signaling pathways involved in cell migration and invasion. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting Dock1 protein in human samples, making it suitable for Western blot applications.Dock1, also known as dedicator of cytokinesis 1, plays a crucial role in mediating cell adhesion and migration processes, making it a potential target for cancer research and studies on cell motility.
By targeting Dock1 with this antibody, researchers can investigate its role in various cellular processes and pathways, ultimately leading to a better understanding of its function in cancer progression and metastasis.With its reliable performance and versatility in detecting Dock1 protein in different cell types, the Dock1 Polyclonal Antibody is an essential tool for researchers aiming to unravel the mechanisms underlying cell migration, invasion, and metastasis in cancer biology and other related fields.
| Antibody Name: | DOCK1 Antibody (PACO54486) |
| Antibody SKU: | PACO54486 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Dedicator of cytokinesis protein 1 protein (1676-1865AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human lung tissue using PACO54486 at dilution of 1:100. |
 | Immunofluorescence staining of Hela cells with PACO54486 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunofluorescence staining of A549 cells with PACO54486 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1. |
| Synonyms: | Dedicator of cytokinesis protein 1 (180 kDa protein downstream of CRK) (DOCK180), DOCK1 |
| UniProt Protein Function: | DOCK1: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1. Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 and BAI1. Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon. Belongs to the DOCK family.Protein type: Cytoskeletal; GEFs, Rac/Rho; GEFs; Adaptor/scaffold; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 10q26.13-q26.3Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosolMolecular Function: protein binding; guanyl-nucleotide exchange factor activity; SH3 domain binding; GTPase activator activityBiological Process: integrin-mediated signaling pathway; axon guidance; cell migration; apoptosis; small GTPase mediated signal transduction; innate immune response; hemopoietic progenitor cell differentiation; phagocytosis, engulfment; blood coagulation; signal transduction; vascular endothelial growth factor receptor signaling pathway; positive regulation of GTPase activity |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
| UniProt Code: | Q14185 |
| NCBI GenInfo Identifier: | 589811536 |
| NCBI Gene ID: | 1793 |
| NCBI Accession: | NP_001277152.1 |
| UniProt Secondary Accession: | Q14185,A9Z1Z5 |
| UniProt Related Accession: | Q14185 |
| Molecular Weight: | |
| NCBI Full Name: | dedicator of cytokinesis protein 1 isoform 1 |
| NCBI Synonym Full Names: | dedicator of cytokinesis 1 |
| NCBI Official Symbol: | DOCK1Â Â |
| NCBI Official Synonym Symbols: | ced5; DOCK180Â Â |
| NCBI Protein Information: | dedicator of cytokinesis protein 1; 180 kDa protein downstream of CRK; DOwnstream of CrK |
| UniProt Protein Name: | Dedicator of cytokinesis protein 1 |
| UniProt Synonym Protein Names: | 180 kDa protein downstream of CRK; DOCK180 |
| Protein Family: | Dedicator of cytokinesis protein |
| UniProt Gene Name: | DOCK1Â Â |
| UniProt Entry Name: | DOCK1_HUMAN |
| Antibodies |
| DOCK1 Antibody (PACO03576) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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