The DOCK1 Antibody (PAC019579) is a valuable tool for researchers studying DOCK1, a protein involved in cellular signaling and immune cell function. This polyclonal antibody is raised in rabbits and is highly specific for human samples, making it ideal for use in Western blot applications. By binding to the DOCK1 protein, this antibody enables the detection and analysis of DOCK1 in various cell types, providing important insights into its role in immune regulation and cancer biology.DOCK1, also known as Dedicator of Cytokinesis 1, is a key player in the regulation of immune cell activation and migration, making it a potential target for therapeutic interventions in conditions such as cancer and immune-related disorders.
By studying the function of DOCK1, researchers can gain a better understanding of how immune cells respond to stimuli and how abnormalities in this process can lead to disease.Overall, the DOCK1 Antibody (PAC019579) is a valuable tool for researchers interested in unraveling the complexities of immune cell signaling and its implications for health and disease. Its specificity and reliability make it an essential component of any study investigating the role of DOCK1 in immune regulation and cancer biology.
Antibody Name:
DOCK1 Antibody (PACO19579)
Antibody SKU:
PACO19579
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human DOCK1
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using PACO19579(DOCK1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO19579(DOCK1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene product binds to the SH3 domain of CRK protein. It may regulate cell surface extension and may have a role in the cell surface extension of an engulfing cell around a dying cell during apoptosis.
Synonyms:
dedicator of cytokinesis 1
UniProt Protein Function:
DOCK1: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1. Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 and BAI1. Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon. Belongs to the DOCK family.Protein type: Cytoskeletal; GEFs, Rac/Rho; GEFs; Adaptor/scaffold; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 10q26.13-q26.3Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosolMolecular Function: protein binding; guanyl-nucleotide exchange factor activity; SH3 domain binding; GTPase activator activityBiological Process: integrin-mediated signaling pathway; axon guidance; cell migration; apoptosis; small GTPase mediated signal transduction; innate immune response; hemopoietic progenitor cell differentiation; phagocytosis, engulfment; blood coagulation; signal transduction; vascular endothelial growth factor receptor signaling pathway; positive regulation of GTPase activity
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
