The DOC2B Polyclonal Antibody (PAC048598) is a valuable tool for researchers studying DOC2B, a calcium sensor protein involved in regulating neurotransmitter release. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the DOC2B protein, researchers can accurately detect and analyze its expression in various cell types, making it ideal for investigations in neuroscience and synaptic transmission research.
DOC2B is a key player in the process of synaptic vesicle fusion, a crucial step in neurotransmitter release at synapses. Its role in regulating calcium-dependent exocytosis makes it a vital target for studies on neuronal communication and synaptic plasticity. Understanding the function of DOC2B is essential for advancing knowledge of neurological disorders and developing potential treatments that target synaptic dysfunction.
Immunohistochemistry of paraffin-embedded human placenta tissue using PACO48598 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO48598 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes (By similarity).
Synonyms:
Double C2-like domain-containing protein β (Doc2-beta), DOC2B, DOC2BL
UniProt Protein Function:
DOC2B: Calcium sensor which positively regulates SNARE- dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes.Protein type: Lipid-bindingChromosomal Location of Human Ortholog: 17p13.3Cellular Component: SNARE complex; synaptic vesicle; cytoplasm; plasma membraneMolecular Function: calcium-dependent phospholipid binding; transporter activityBiological Process: positive regulation of calcium ion-dependent exocytosis; protein localization; calcium ion-dependent exocytosis of neurotransmitter; positive regulation of insulin secretion; positive regulation of vesicle fusion
UniProt Protein Details:
NCBI Summary:
There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2B is expressed ubiquitously and is suggested to be involved in Ca(2+)-dependent intracellular vesicle trafficking in various types of cells. [provided by RefSeq, Jul 2008]
