The DNMT3L Polyclonal Antibody (CAB13591) is a valuable tool for researchers studying DNA methyltransferase 3-like protein (DNMT3L), which plays a crucial role in DNA methylation and gene regulation. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to DNMT3L, researchers can accurately detect and analyze this protein in various cell types, making it an ideal choice for studies in epigenetics, developmental biology, and cancer research.
DNMT3L is known to interact with DNMT3A and DNMT3B, contributing to the establishment of DNA methylation patterns during embryonic development and gametogenesis. Dysregulation of DNMT3L has been implicated in various diseases, including cancer and neurological disorders, making it a promising target for therapeutic intervention. By understanding the function and regulation of DNMT3L, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and potentially develop novel treatment strategies.
Product Name:
DNMT3L Rabbit Polyclonal Antibody
SKU:
CAB13591
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 151-386 of human DNMT3L (NP_787063.1).
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
29947
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DNMT3L antibody (CAB13591) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
