The DNMT3B Polyclonal Antibody (CAB7239) is a valuable tool for researchers studying DNMT3B, a DNA methyltransferase enzyme essential for epigenetic regulation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to DNMT3B, the antibody allows for the detection and analysis of DNMT3B expression in various cell types, making it ideal for studies in epigenetics, cancer research, and developmental biology.DNMT3B plays a crucial role in establishing and maintaining DNA methylation patterns, which are important for gene regulation and chromatin structure.
Dysregulation of DNMT3B has been implicated in various diseases, including cancer, neurological disorders, and developmental abnormalities. Understanding the function of DNMT3B is essential for identifying potential therapeutic targets and developing treatments for these conditions.Overall, the DNMT3B Polyclonal Antibody provides researchers with a reliable tool for investigating the role of DNMT3B in epigenetic regulation and disease pathology. Its high specificity and sensitivity make it a valuable asset for those studying DNA methylation dynamics and the impact of epigenetic changes on gene expression.
Product Name:
DNMT3B Rabbit Polyclonal Antibody
SKU:
CAB7239
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant protein of human DNMT3B
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:
ICF; ICF1; FSHD4; M.HsaIIIB; DNMT3B
Positive Sample:
Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
96kDa
Observed MW:
96kDa
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Purification Method:
Affinity purification
Gene ID:
1789
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Rat testis, using DNMT3B Rabbit pAb (CAB7239) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
