The DNMT3B Polyclonal Antibody (CAB2899) is a valuable tool for researchers studying DNA methyltransferase 3B (DNMT3B), an enzyme involved in DNA methylation and gene regulation. This antibody, produced in rabbits, is highly specific to human DNMT3B and has been validated for use in Western blot applications.DNMT3B plays a crucial role in establishing and maintaining DNA methylation patterns, which are essential for gene expression and cellular function. Dysregulation of DNMT3B has been implicated in various diseases, including cancer and neurological disorders. By targeting DNMT3B with this antibody, researchers can investigate its expression levels and localization in different cell types, providing insights into its function and potential therapeutic implications.
With its high reactivity and specificity, the DNMT3B Polyclonal Antibody (CAB2899) is a valuable tool for studying epigenetic regulation and its role in disease development. Whether exploring the mechanisms of cancer progression or investigating neurodevelopmental disorders, this antibody is essential for conducting cutting-edge research in the fields of genetics and molecular biology.
Product Name:
DNMT3B Rabbit Polyclonal Antibody
SKU:
CAB2899
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 345-475 of human DNMT3B (NP_008823.1).
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Purification Method:
Affinity purification
Gene ID:
1789
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNMT3B Rabbit pAb (CAB2899) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
