The DNMT3B Polyclonal Antibody (CAB11079) is an essential tool for studying DNA methyltransferase 3B (DNMT3B), a key enzyme involved in DNA methylation and gene regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By specifically binding to DNMT3B protein, it enables accurate detection and analysis in a variety of cell types, making it ideal for research in epigenetics, cancer biology, and developmental biology.DNMT3B plays a crucial role in the establishment and maintenance of DNA methylation patterns, which are essential for gene expression and genomic stability.
Dysregulation of DNMT3B has been linked to various diseases, including cancer, neurological disorders, and developmental abnormalities. Therefore, studying the function and regulation of DNMT3B is essential for advancing our understanding of these conditions and developing targeted therapies.With its high specificity and sensitivity, the DNMT3B Polyclonal Antibody (CAB11079) is a valuable tool for researchers investigating the role of DNA methylation in health and disease. Its applications extend to a wide range of biological studies, making it a versatile and reliable reagent for laboratory research.
Product Name:
DNMT3B Rabbit Polyclonal Antibody
SKU:
CAB11079
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant Protein corresponding to a sequence within amino acids 1-200 of human DNMT3B (NP_008823.1).
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Purification Method:
Affinity purification
Gene ID:
1789
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNMT3B Rabbit pAb (CAB11079) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
