The DNMT3A Polyclonal Antibody (CAB2065) is a valuable tool for researchers studying DNA methyltransferase 3A (DNMT3A), an enzyme involved in DNA methylation and gene regulation. This antibody is raised in rabbits and is highly reactive with human DNMT3A protein samples.DNMT3A plays a critical role in the establishment of DNA methylation patterns during development and has been implicated in various diseases, including cancer and neurological disorders. The DNMT3A Polyclonal Antibody is validated for use in Western blot applications, enabling researchers to detect and analyze DNMT3A levels in different cell types.
By targeting DNMT3A, researchers can gain insights into its function in epigenetic regulation and its potential role in disease development. This antibody is a valuable tool for studies in cancer research, epigenetics, and developmental biology, providing researchers with the ability to further understand the mechanisms underlying these complex biological processes.
Product Name:
DNMT3A Rabbit Polyclonal Antibody
SKU:
CAB2065
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human DNMT3A (NP_072046.2).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
TBRS; HESJAS; DNMT3A2; M.HsaIIIA; DNMT3A
Positive Sample:
A-549,293T,HeLa,HL-60,MCF7,HepG2,Mouse testis
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
102kDa
Observed MW:
110kDa/130kDa
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Purification Method:
Affinity purification
Gene ID:
1788
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNMT3A Rabbit pAb (CAB2065) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
