The DNMT1 Polyclonal Antibody (CAB17474) is a valuable tool for researchers studying DNA methylation and epigenetics. This antibody, generated in rabbits, specifically targets DNA methyltransferase 1 (DNMT1) protein in human samples and is suitable for use in Western blot applications.DNMT1 is a key enzyme involved in maintaining DNA methylation patterns during cell division, playing a crucial role in gene regulation and epigenetic inheritance. Aberrant DNMT1 activity has been linked to various diseases, including cancer, neurological disorders, and developmental abnormalities.
By using the DNMT1 Polyclonal Antibody, researchers can accurately detect and analyze DNMT1 protein levels in various cell types, providing insights into the role of DNA methylation in health and disease. This antibody is an essential tool for studies exploring the epigenetic regulation of gene expression and potential therapeutic targets in various pathologies.
Product Name:
DNMT1 Rabbit Polyclonal Antibody
SKU:
CAB17474
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 760-1109 of DNMT1 (NP_001370.1).
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1786
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using DNMT1 Rabbit pAb (CAB17474) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
